Simple, rapid, sensitive and accurate and reproducible reverse phase high performance liquid chromatographic (RP-HPLC) method was developed for estimation of Tapentadol Hydrochloride. Chromatography was carried out at ambient temperature and separation of this drug was achieved on C18 column (250*4.6mm*5µm) as stationary phase with a mobile phase comprising of methanol: water (70:30, pH-5), at flow rate 1.5 mL/min. The detection wavelength for tapentadol hydrochloride was 272 nm. The retention time of tapentadol hydrochloride 2.38 minute. The Linearity of tapentadol hydrochloride was in the range of 20-100 μg/ml (r2=0.997). The results of analysis have been validated statistically for linearity, LOD, LOQ, accuracy, precision and recovery studies of the proposed method. Present methods are economical and sensitive for the estimation of Tapentadol Hydrochloride in bulk and tablet dosage forms in routine manner.
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